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Isolation and determination of deoxynivalenol by reversed-phase high-pressure liquid chromatography

Submitted by webadmin on Mon, 10/17/2016 - 16:13
Pharmaceutical Methods,2011,2,1,25-29.
Published:January 2011
Type:Original Article

Isolation and determination of deoxynivalenol by reversed-phase high-pressure liquid chromatography

Vikas Kumar Gupta, Pronobesh Chattopadhyay, Mohan Ch Kalita1, Asshwani Kumar Chaurasia, Hemanta Kumar Gogoi, Lokendra Singh

Division of Biotechnology, Defense Research Laboratory, Post Bag No: 2, Tezpur-784 001,

1Gauhati University, Department of Biotechnology, Guwahati-781 014, Assam, India

Abstract:

Deoxynivalenol (DON) is a mycotoxin produced by food contamination. It is a pharmacologically active compound that acts on the serotonin receptor, leading to several neuroendocrine and hematological disorders. In this article we describe a simple, accurate, and sensitive method for the quantification of DON. DON was quantified using a Phenomenex® ODS analytical C18 column (150 mm × 46 mm, 5 μm) with a mobile phase composed of mixture of water-acetonitrile-methanol (5:4:1, v/v/v) at a flow rate of 1.5 ml/min and at 254 nm in an ultraviolet (UV) detector The method has been validated with isolated samples of DON and provides a tool for the control of substandard and counterfeit commercial food products.

Structure of deoxynivalenol