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Spectrophotometric and High Performance Liquid Chromatographic Determination of Amlodipine Besylate and Nebivolol Hydrochloride in Tablets Dosage Form

Submitted by webadmin on Tue, 10/18/2016 - 12:02
Pharmaceutical Methods,2016,7,1,1-7.
Published:January 2016
Type:Original Article

Spectrophotometric and High Performance Liquid Chromatographic Determination of Amlodipine Besylate and Nebivolol Hydrochloride in Tablets Dosage Form

Madhuri Ajay Hinge, Jigar Arjunbhai Patel, Rajvi J. Mahida

Department of Quality Assurance, ROFEL Shri G.M. Bilakhia College of Pharmacy, Vapi, Gujarat, INDIA.

Abstract:

Introduction: Absorbance ratio spectrophotometric method and high performance liquid chromatographic method were developed for the determination of the Amlodipine Besylate and Nebivolol Hydrochloride in tablet dosage form. Methods: Absorbance ratio spectrophotometric method and high performance liquid chromatographic method were developed for the determination of the Amlodipine Besylate and Nebivolol Hydrochloride in tablet dosage form. A simple liquid chromatographic assay has been developed for the determination of Amlodipine Besylate and Nebivolol Hydrochloride. A C18 (250×4.6 mm, 5 μ) column was used with a mobile phase consisting of Water: Acetonitrile ( pH adjusted to 3.5 with ortho phosphoric acid) at a flow rate of 1.0 ml min-1. Quantitation was achieved with UV detection at 268 nm based on the peak height ratios. Results: Calibration curves were linear in the range of 10–30 μg/ml for Amlodipine Besylate and 10-30 μg/ml for Nebivolol Hydrochloride in absorbance ratio method. Correlation coefficient found to be close to 0.9995 for both the drugs. Accuracy for both the drugs was in the range of 99-101%. Beer’s law was obeyed in a concentration range of 10-30 mg ml-1 for Amlodipine Besylate and 10-30 mg ml-1 for Nebivolol Hydrochloride and the regression line equation was derived with a correlation coefficient of 0.9999 and 0.9998 for Amlodipine Besylate and Nebivolol Hydrochloride respectively. Discussion: The proposed procedures were successfully applied to the determination of Amlodipine Besylate and Nebivolol Hydrochloride in bulk and tablet form, with high percentage of recovery, good accuracy and precision.

Calibration curve of AML at 268 nm.