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New Spectrophotometric Methods for the Simultaneous Determination of Irbesartan and Hydrochlorothiazide in Combined Dosage Forms

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Pharmaceutical Methods,2015,6,3,120-125.
Published:July 2015
Type:Original Article

New Spectrophotometric Methods for the Simultaneous Determination of Irbesartan and Hydrochlorothiazide in Combined Dosage Forms

Josyula Sai Phani Kumar and Mukthinuthalapati Mathrusri Annapurna*

Department of Pharmaceutical Analysis and Quality Assurance, GITAM Institute of Pharmacy, GITAM University, Visakhapatnam, INDIA.

Abstract:

The combination of Irbesartan and Hydrochlorothiazideis mainly used for the treatment of hypertension. New spectrophotometric methods have been developed and validated for the simultaneous determination of Irbesartan and Hydrochlorothiazide in pharmaceutical formulations (Tablets) by two methods i.e. simultaneous equation method and absorbance ratio method (Q‐analysis) in phosphate buffer (pH 7.5). Irbesartan and Hydrochlorothiazide have shown linearity over the concentration range 5-35 μg/ml and 0.2-40 μg/ml in both the methods.

Overlay absorption Spectrum of IRB (20 μg/ml), HTZ (20 μg/ml) and mixture of IRB+HTZ (Formulation) in phosphate buffer pH-7.5