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An Overview of Liquid Chromatography-Mass Spectroscopy Instrumentation

Submitted by webadmin on Wed, 10/19/2016 - 11:32
Pharmaceutical Methods,2014,5,2,47-55.
Published:July 2014
Type:Review Article

An Overview of Liquid Chromatography-Mass Spectroscopy Instrumentation

Subramani Parasuraman1*, Anish R2, Subramani Balamurugan3, Selvadurai Muralidharan4, Kalaimani Jayaraj Kumar5 and Venugopal Vijayan5

1Unit of Pharmacology, Faculty of Pharmacy, AIMST University, Bedong 08100, Kedah, Malaysia.

2Gulf Pharmaceutical Industries (Julphar), United Arab Emirates.

3Department of Pharmacology, College of Pharmacy, Madras Medical College, Chennai, India.

4Unit of Pharmaceutical Chemistry, Faculty of Pharmacy, AIMST University, Bedong 08100, Kedah, Malaysia.

5Unit of Pharmaceutical Technology, Faculty of Pharmacy, AIMST University, Bedong 08100, Kedah, Malaysia.

Abstract:

Chromatography is a separation technique used to separate the individual compound from a mixture using a stationary and mobile phase. Discovery of chromatography is a millstone event in biomedical research. Chromatographic separation is based on the principles of adsorption, partition, ion exchange, molecular exclusion, affinity and Chirality. There are many types of chromatography available for quantitative and qualitative analysis of pharmaceutical agents, which includes Liquid Chromatography-Mass Spectrometry (LC-MS). Combination of chromatography with spectrometry is first reported in 1967 and first LC-MS system was introduced in 1980s. LC-MS is an analytical chemistry technique that combines the physical separation capabilities of liquid chromatography with the mass analysis and mass spectrometry. Mainly the LC-MS contains liquid chromatography assembly, ion generation unit/ ionization source, mass analyzer and mass spectrometric data acquisition. LC-MS is most commonly used in biomedical sciences for pharmacokinetic analysis, genetic analysis, structural elucidation, etc. The main objective of this review is to overview the principle, instrumentation and application of LC-MS.

Instrumentation of LC-MS