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Development and validation of HPTLC method for the determination of mycophenolate mofetil in bulk and pharmaceutical formulation

Submitted by webadmin on Wed, 10/26/2016 - 12:13
Pharmaceutical Methods,2012,3,2,90-93.
Published:December 2012
Type:Original Article

Development and validation of HPTLC method for the determination of mycophenolate mofetil in bulk and pharmaceutical formulation

S. Kathirvel, K. Rajendra Prasad, K. Madhu Babu

Department of Pharmaceutical analysis, Hindu College of Pharmacy, Amaravathi road, Guntur, A. P, India

Abstract:

Aim: Described in this manuscript is the first ever reported, new, simple, highperformance thin-layer chromatographic method for the determination of mycophenolate mofetil in bulk and tablet dosage form. Materials and Methods: The drug was separated on aluminum plates precoated with silica gel 60 F254 with toluene, acetone, and methanol in the ratio of 6:2:2 (v/v/v) as the mobile phase. Quantitative analysis was performed by densitometric scanning at 254 nm. Results: The method was validated for linearity, accuracy, precision, and robustness. The calibration plot was linear in the range of 100–500 ng band-1 for mycophenolate mofetil. The method was successfully applied to the analysis of the drug in a pharmaceutical dosage form.