Standardization and Phytochemical Evaluation of Spray-dried Powder of Fruit Juice of Emblica officinalis Gaertn
Introduction: The fruits of Emblica officinalis Gaertn. (Family: Euphorbiaceae) have been reported to contain constituents with variable biological activity. The present investigation was carried out to standardize the formulation prepared by spray-dried powder of fruit juice of E. officinalis. Materials and Methods: Standardization was carried out by determination of physicochemical parameters, presence or absence of heavy metals, screening for phytochemicals and development of HPTLC fingerprint for presence of gallic acid and quercetin. Results: The percentage of moisture content, total ash, acid insoluble ash, water-soluble ash and alcohol soluble extractive, water soluble extractive, chloroform soluble extractive of spray dried powder were found to be 3.2±0.25, 6.7±0.17, 3.1±0.10, 5.1±0.22, 25.21±0.16, 35.21±0.21 and 5.1±0.22, respectively. The concentrations of all the tested heavy metals were below the WHO acceptable limits. Preliminary phytochemical analysis indicated presence of carbohydrates, alkaloids, anthraquinone glycoside, mucilage and gums, proteins and amino acids in very small amount and tannins, flavonoids were found to be present in maximum amount and HPTLC fingerprint clearly demonstrated the presence of gallic acid (9.5-10.5%w/w). Conclusion: In conclusion, the results obtained from this study can be used to standardize the formulations prepared by spraydried powder of E. officinalis.