HPLC for Peptides and Proteins: Principles, Methods and Applications
Peptides and proteins are large biomolecules composed of long chains of L -amino acids and plays key functions in the living organisms. A variety of techniques are used to characterize these biomolecules and, among the techniques used, high performance liquid chromatography (HPLC) has been extensively employed both for the preparative as well as the analytical characterization of peptides and proteins. Various HPLC techniques developed for the separation of proteins rely on the differences in the adsorption characteristics, surface charge, ligand specificity, and molecular size of protein molecules. Advancements made in these HPLC techniques have contributed immensely to the development of peptides and proteins based pharmaceuticals. Present article summarizes the principle, methods, and applications of the most common HPLC techniques used in the field of peptides and proteins.