A modified TLC bioautographic technique for the detection of antilithiatic potential of therapeutic plants from Indigenous Ayurvedic System

Abstract

Author(s): Ankit Subhash Kale, Anita Surendra Patil, and Hariprasad Madhukarrao Paikrao

Introduction: Urolithiasis is the most painful disorder associated with formation of stone in the urinary system. In Indian Ayurvedic systems, medicinal plants are preferred as natural drug resources. In the present study aqueous extract of Bryophyllum pinnatum (L.), Tribulus terrestris (L.), Phyllanthus niruri (L.), Abutilon indicum (L.) and positive control tri-sodium citrates were used to investigate their antilithiatic potential in the modified dot blot assay. Methods: In the present study aqueous extract of all four plants in variable concentrations were tested for antilithiatic potential using dot blot method. In this method glass plates were overlaid by 0.8% agar containing 0.2 M CaCl2. These plates were deepening in 0.2 M Ammonium chlorite and then deepen in Alizarin Red S and Arsenzo III. Results: After removal of plate from the coloring solutions, zones of inhibition were observed, which shows the maximum CaOx crystallization percent inhibition of B. pinnatum (73.16 ± 2.05 %), followed by Phyllanthus niruri (L.), Abutilon indicum (L.) and Tribulus terrestris (L.) on TLC bioautography based dot blot assay. The lowest IC50 (70.163 mg/ml) of B. pinnatum confirms its higher antilithiatic potential as compared to rest of three plants. Conclusion: We conclude that aqueous B. pinnatum extract (100 mg/ml) showed maximum CaOx crystallization percent inhibition (73.16 ± 2.05 %), which was followed by Phyllanthus niruri (L.), Abutilon indicum (L.) and Tribulus terrestris (L.) on TLC bioautography based dot blot assay. The lowest IC50 (70.163 mg/ml) of B. pinnatum the confirms its higher antilithiatic potential as compared to rest of the three plants.

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