Pharmaceutical Methods
Publishing Quality Research & Reviews
Pharmaceutical Methods
Publishing Quality Research & Reviews
Author(s): Sumanta Mondal, Goluguri Sunil Reddy, Prasenjit Mondal, Vadlapati Sheeba Prathyusha, Aishwarya P Nair, Syed Tazib Rahaman
Background: The present research paper described about the optimization of various zero order and first order UV spectrophotometric method using different buffers. Validation study was performed to develop a simple, sensitive, rapid, accurate and economical Ultra Violet spectrophotometric method for the estimation of Valganciclovir.
Methods: UV 1800 double beam UV Visible Spectrophotometer with a pair of 10mm path length matched quartz cells were used for the study. Method A (Water), Method B (phosphate buffer pH2), Method C (Phosphate buffer pH4) and Method D (phosphate buffer pH5) were developed for estimation of Valganciclovir by zero-order and first-order derivative.
Results: Linearity was carried out in the concentration range of 5-60 µg/ml and the correlation coefficient were found to be 0.999. The percentage recoveries were found to be 98-102%. The relative standard deviation was found to be <2%. The LOD and LOQ were found to be 0.3241 µg/ml and 0.8227 µg/ml respectively.
Conclusion: Hence, the methods were validated according to ICH guidelines and can be adopted for the routine analysis of Valganciclovir in pure and tablet dosage form.
Pharmaceutical Methods received 3403 citations as per google scholar report
