Effect of sample Concentration on the Characterization of Liposomes using Dynamic light Scattering Technique

Abstract

Author(s): Ramesh Surianarayanan, Hosakote Gurumallappa Shivakumar, Naga Sravan Kumar Varma Vegesna, and Atul Srivastava

The objective of the study was to assess the effect of liposomal concentration on the characterization of liposome using dynamic light scattering technique. Method: Liposome of a water soluble active, Niacinamide, was taken for the study. Phospholipid combination (INCI name lecithin and Propane diol) was used to form liposomes of Niacinamide. Concentrated liposomal suspension was prepared using various ratios of phospholipid. The concentrated liposomal suspension was diluted five times using distilled water. Concentrated liposomal suspension and diluted suspension were characterized for particle size, PDI, conductivity and Zeta potential. Their surface morphology was studied with SEM images. Results: The data on conductivity, PDI, zeta potential and particle size were significantly different between concentrated and diluted liposomal samples. The use of various phospholipid ratios appears to significantly affect PDI, zeta potential and particle size in concentrated sample. The data on diluted samples indicated that the phosholipid level has no significant effect on these parameters. Conclusion: In conclusion, characterizing liposomes using techniques which involves dynamic light scattering, that is widely used, liposome concentration during measurement is important to get reliable results.

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