Pharmaceutical Methods
Publishing Quality Research & Reviews
Pharmaceutical Methods
Publishing Quality Research & Reviews
Author(s): Preeti Sudhir Bobade, and Saurabh Baburaoji Ganorkar
The amalgamation of a unique spectrophotometric technique and principle concept of studying variations in pharmaceutical brands as a contrivance for simultaneous determination of the highly marketed combination of Bisoprolol fumarate and Hydrochlorothiazide is established hereby. The commercial purpose of marketing same drugs or drug combinations by different names creates scope for such analysis. Hence an accurate, precise and sensitive Q-Absorbance ratio UV-spectrophotometric method has been developed and validated for the simultaneous estimation of Bisoprolol Fumarate (BF) and Hydrochlorothiazide (HCT) in combined pharmaceutical dosage form. The analysis was performed using 0.1 N NaOH as solvent. Q-Absorbance ratio method involves formation of Q-absorbance equation at two wavelengths 274 nm (?max of HCT) and 238.4 nm (iso-absorptive point). Calibration curves were linear in range of 8-96 μg/mL (r2=0.999, 0.999) and 4-48 μg/mL (r2= 0.998, 0.999) for BF and HCT respectively. The method was found to be rapid, economical and rugged as indicated by low values of percent RSD, can successfully be applied for the routine analysis of BF and HCT in bulk and combined pharmaceutical dosage form.
