HPTLC Method Development and Validation of Cilnidipine and Metoprolol Succinate in Combined Dosage Form

Abstract

Author(s): Dhwani Desai, Nirmal Vashi, Hitesh Dalvadi, Shuchi Desai, and Madhuri Hinge

Introduction and Objectives: The present work involves development and validation of HPTLC method for simultaneous estimation of Cilnidipine and Metoprolol Succinate in their combined tablet dosage form. Method: In HPTLC method, Silica Gel G60 F254 TLC plate as the stationary phase and a mobile phase of Toluene: Chloroform: Methanol: Glacial acetic acid (45: 25: 25: 5 v/v/v/v) was used to resolve CIL and METO. CIL and METO were quantified at 231 nm. The proposed method weas validated according to International Conference on Harmonization. Result and Discussion: Two well-separated and sharp peak for CIL and METO were obtained at R_f values of 0.70 ± 0.01 and 0.34 ± 0.005 respectively. The linearity range obtained for HPTLC method were 100-500 ng/spot and 500-2500 ng/spot for CIL and METO respectively. Conclusion: Method validation was found to be accurate, specific and precise.The developed method was successfully applied for estimation of CIL and METO in combined tablet formulation.

• Latest

A Novel Rapid Approach for the Estimation of Ketoconazole Using Reverse Phase Ultra Performance Liquid Chromatography in Bulk and Tablet Dosage from

Analytical Validation and Stability Indicating Studies for Simultaneous Estimation of Benidepine and Metoprolol by Strong Cation Exchange (SCX) Chromatography

Validated RP-HPLC Method Development of Pazopanib in Bulk and its Pharmaceutical Dosage Form