Pharmaceutical Methods
Publishing Quality Research & Reviews
Pharmaceutical Methods
Publishing Quality Research & Reviews
Author(s): Vipin Prakash, Jerad Suresh, Sarif Niroush Konari
Introduction: Aztreonam belongs to the monobactam class of antibiotic used to treat infectious diseases produced by gram-negative bacteria. This work presents the development of LC methods for the estimation of Titled drug in pharmaceutical dosage form and forced degradation studies on four different stress conditions.
Method: A waters HPLC Inspire (4.6 x 250mm, 5µm) in isocratic mode, with mobile phase containing buffer: Acetonitrile (40:60 %v/v) pH 3 adjusted with orthophosphoric acid were used. The flow rate was 1ml/min and linearity range was established at 5- 25 µg/ml.
Conclusion: Degradation studies disclose method abilities on various stress conditions. Forced degradation results can be used for the development of stable dosage form and for the designing of proper storage requirement. The proposed method is accurate, precise, specific and rapid for the estimation of aztreonam injection
